BAIT

TED3

ARABIDOPSIS PEROXIN 2, ATPEX2, F20B17.23, F20B17_23, PEROXIN 2, PEX2, PEX2P, REVERSAL OF THE DET PHENOTYPE 3, AT1G79810
peroxin-2
Arabidopsis thaliana (Columbia)
PREY

HY5

BZIP TRANSCRIPTION FACTOR HY5, ELONGATED HYPOCOTYL 5, F2I11.150, F2I11_150, REVERSAL OF THE DET PHENOTYPE 5, TED 5, AT5G11260
transcription factor HY5
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Ectopic expression of the RING domain of the Arabidopsis peroxin2 protein partially suppresses the phenotype of the photomorphogenic mutant de-etiolated1.

Desai M, Kaur N, Hu J

The Arabidopsis constitutive photomorphogenic/de-etiolated 1/FUSCA (COP/DET1/FUS) proteins repress photomorphogenesis by degrading positive regulators of photomorphogenesis, such as the transcription factor long hypocotyl5 (HY5). The gain-of-function mutant ted3, which partially suppresses the det1 mutant, contains a missense mutation of a Val-to-Met substitution before the C-terminal RING finger domain of the peroxisomal membrane protein peroxin2 (PEX2). We hypothesized that a truncated PEX2 ... [more]

PLoS ONE Sep. 24, 2014; 9(9);e108473 [Pubmed: 25248106]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TED3 HY5
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Low-BioGRID
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Curated By

  • BioGRID