An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The cystic fibrosis transmembrane conductance regulator interacts with and regulates the activity of the HCO3- salvage transporter human Na+-HCO3- cotransport isoform 3.

Park M, Ko SB, Choi JY, Muallem G, Thomas PJ, Pushkin A, Lee MS, Kim JY, Lee MG, Muallem S, Kurtz I

Cystic fibrosis transmembrane conductance regulator (CFTR) regulates both HCO(3)(-) secretion and HCO(3)(-) salvage in secretory epithelia. At least two luminal transporters mediate HCO(3)(-) salvage, the Na(+)/H(+) exchanger (NHE3) and the Na(+)-HCO(3)(-) cotransport (NBC3). In a previous work, we show that CFTR interacts with NHE3 to regulate its activity (Ahn, W., Kim, K. W., Lee, J. A., Kim, J. Y., Choi, ... [more]

J. Biol. Chem. Dec. 27, 2002; 277(52);50503-9 [Pubmed: 12403779]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

SLC9A3R1

Gene Ontology Biological Process

Gene Ontology Molecular Function

PDZ domain binding [IPI]beta-2 adrenergic receptor binding [IPI]beta-catenin binding [IPI]chloride channel regulator activity [IDA]growth factor receptor binding [IPI]phosphatase binding [IPI]protein binding [IPI]protein self-association [IDA]receptor binding [IPI]

Gene Ontology Cellular Component

SLC4A7