An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomic analysis and identification of cellular interactors of the giant ubiquitin ligase HERC2.

Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, Harper JW, Howley PM

HERC2 is a large E3 ubiquitin ligase with multiple structural domains that has been implicated in an array of cellular processes. Mutations in HERC2 are linked to developmental delays and impairment caused by nervous system dysfunction, such as Angelman Syndrome and autism-spectrum disorders. However, HERC2 cellular activity and regulation remain poorly understood. We used a broad proteomic approach to survey ... [more]

J. Proteome Res. Feb. 06, 2015; 14(2);953-66 [Pubmed: 25476789]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

HERC2

Gene Ontology Biological Process

Gene Ontology Molecular Function

SUMO binding [IDA]guanyl-nucleotide exchange factor activity [NAS]protein binding [IPI]ubiquitin protein ligase binding [IPI]ubiquitin-protein transferase activity [IDA]

Gene Ontology Cellular Component

EXD2