BAIT
NEK2
AA617254, C77054
NIMA (never in mitosis gene a)-related expressed kinase 2
GO Process (10)
GO Function (4)
GO Component (9)
Gene Ontology Biological Process
- blastocyst development [IMP]
- centrosome separation [ISO]
- chromosome segregation [IMP, ISO]
- mitotic sister chromatid segregation [IMP]
- negative regulation of DNA binding [IDA]
- negative regulation of centriole-centriole cohesion [ISO]
- protein autophosphorylation [ISO]
- protein phosphorylation [IBA, IDA, ISO]
- regulation of attachment of spindle microtubules to kinetochore [ISO]
- spindle assembly involved in mitosis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
CDCA3
GRCC8, TOME-1
cell division cycle associated 3
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances.
The organization of a cell emerges from the interactions in protein networks. The interactome is critically dependent on the strengths of interactions and the cellular abundances of the connected proteins, both of which span orders of magnitude. However, these aspects have not yet been analyzed globally. Here, we have generated a library of HeLa cell lines expressing 1,125 GFP-tagged proteins ... [more]
Cell Oct. 22, 2015; 163(3);712-23 [Pubmed: 26496610]
Throughput
- High Throughput
Additional Notes
- interaction detected by quantitative BAC-GFP interactomics (QUBIC)
Curated By
- BioGRID