BAIT

ORF4

HHV8GK18_gp02
complement control protein
GO Process (0)
GO Function (0)
GO Component (0)
Human Herpesvirus 8
PREY

CAND1

TIP120, TIP120A
cullin-associated and neddylation-dissociated 1
UPS Project
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Global mapping of herpesvirus-host protein complexes reveals a transcription strategy for late genes.

Davis ZH, Verschueren E, Jang GM, Kleffman K, Johnson JR, Park J, Von Dollen J, Maher MC, Johnson T, Newton W, Jaeger S, Shales M, Horner J, Hernandez RD, Krogan NJ, Glaunsinger BA

Mapping host-pathogen interactions has proven instrumental for understanding how viruses manipulate host machinery and how numerous cellular processes are regulated. DNA viruses such as herpesviruses have relatively large coding capacity and thus can target an extensive network of cellular proteins. To identify the host proteins hijacked by this pathogen, we systematically affinity tagged and purified all 89 proteins of Kaposi's ... [more]

Mol. Cell Jan. 22, 2015; 57(2);349-60 [Pubmed: 25544563]

Throughput

  • High Throughput

Curated By

  • BioGRID