Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The STUbL RNF4 regulates protein group SUMOylation by targeting the SUMO conjugation machinery.

Kumar R, Gonzalez-Prieto R, Xiao Z, Verlaan-de Vries M, Vertegaal ACO

SUMO-targeted ubiquitin ligases (STUbLs) mediate the ubiquitylation of SUMOylated proteins to modulate their functions. In search of direct targets for the STUbL RNF4, we have developed TULIP (targets for ubiquitin ligases identified by proteomics) to covalently trap targets for ubiquitin E3 ligases. TULIP methodology could be widely employed to delineate E3 substrate wiring. Here we report that the single SUMO ... [more]

Nat Commun Dec. 27, 2016; 8(1);1809 [Pubmed: 29180619]

Throughput

  • High Throughput

Curated By

  • BioGRID