BAIT
KRAS
C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2
Kirsten rat sarcoma viral oncogene homolog
GO Process (16)
GO Function (2)
GO Component (5)
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- MAPK cascade [TAS]
- Ras protein signal transduction [TAS]
- activation of MAPKK activity [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [TAS]
- leukocyte migration [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of cell proliferation [IMP]
- positive regulation of gene expression [IMP]
- positive regulation of protein phosphorylation [IMP]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CHP1
CHP, SLC9A1BP, Sid470p, p22, p24
calcineurin-like EF-hand protein 1
GO Process (24)
GO Function (7)
GO Component (9)
Gene Ontology Biological Process
- cellular response to acidic pH [ISS]
- cytoplasmic microtubule organization [ISS]
- membrane docking [ISS]
- membrane fusion [ISS]
- membrane organization [ISS]
- microtubule bundle formation [ISS]
- negative regulation of NF-kappaB transcription factor activity [IDA]
- negative regulation of calcineurin-NFAT signaling cascade [IDA]
- negative regulation of phosphatase activity [IDA]
- negative regulation of protein autophosphorylation [ISS]
- negative regulation of protein import into nucleus [IDA]
- negative regulation of protein kinase activity [ISS]
- negative regulation of protein phosphorylation [ISS]
- negative regulation of protein ubiquitination [ISS]
- positive regulation of protein glycosylation [ISS]
- positive regulation of protein targeting to membrane [ISS]
- positive regulation of protein transport [ISS]
- positive regulation of sodium:proton antiporter activity [IDA, ISS]
- potassium ion transport [TAS]
- protein export from nucleus [ISS]
- protein stabilization [ISS]
- regulation of intracellular pH [IDA]
- regulation of neuron death [IMP]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
Proximity-dependent biotin labeling (BioID) may identify new targets for cancers driven by difficult-to-drug oncogenes such as Ras. Therefore, BioID was used with wild-type (WT) and oncogenic mutant (MT) H-, K-, and N-Ras, identifying known interactors, including Raf and PI3K, as well as a common set of 130 novel proteins proximal to all Ras isoforms. A CRISPR screen of these proteins for ... [more]
Mol. Cell Feb. 21, 2019; 73(4);830-844.e12 [Pubmed: 30639242]
Throughput
- High Throughput
Additional Notes
- BioID
Curated By
- BioGRID