An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.

Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, Khavari PA

Proximity-dependent biotin labeling (BioID) may identify new targets for cancers driven by difficult-to-drug oncogenes such as Ras. Therefore, BioID was used with wild-type (WT) and oncogenic mutant (MT) H-,Â K-, and N-Ras, identifying known interactors, including Raf and PI3K, as well as a common set of 130 novel proteins proximal to all Ras isoforms. A CRISPR screen of these proteins for ... [more]

Mol. Cell Feb. 21, 2019; 73(4);830-844.e12 [Pubmed: 30639242]

Throughput

High Throughput

Additional Notes

BioID

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
KRAS GNL3L	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Vichas A (2021)	High	-	BioGRID	3344047
KRAS GNL3L	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Luo J (2009)	High	-	BioGRID	1414571

Curated By

BioGRID

Interaction Summary

KRAS

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein complex binding [IDA]

Gene Ontology Cellular Component

GNL3L

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTPase activity [IBA]poly(A) RNA binding [IDA]protein binding [IPI]