RAB27A
Gene Ontology Biological Process
- GTP catabolic process [IBA]
- Rab protein signal transduction [IBA]
- antigen processing and presentation [IMP]
- cellular protein metabolic process [TAS]
- complement-dependent cytotoxicity [IMP]
- exocytosis [IDA]
- exosomal secretion [IMP]
- intracellular protein transport [IBA]
- melanosome localization [IMP]
- melanosome transport [IBA]
- multivesicular body organization [IMP]
- multivesicular body sorting pathway [IMP]
- positive regulation of constitutive secretory pathway [IMP]
- positive regulation of exocytosis [IMP]
- positive regulation of gene expression [IMP]
- positive regulation of phagocytosis [IMP]
- positive regulation of reactive oxygen species biosynthetic process [IMP]
- positive regulation of regulated secretory pathway [IGI, IMP]
- protein secretion [IBA]
- synaptic vesicle transport [TAS]
- vesicle docking involved in exocytosis [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SYTL5
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells.
Slp4-a (synaptotagmin-like protein 4-a)/granuphilin-a is specifically localized on dense-core vesicles in PC12 cells and negatively controls dense-core vesicle exocytosis through specific interaction with Rab27A via the N-terminal Slp homology domain (SHD) (Fukuda, M., Kanno, E., Saegusa, C., Ogata, Y., and Kuroda, T. S. (2002) J. Biol. Chem. 277, 39673-39678). However, the mechanism of the inhibition by Slp4-a has never been ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RAB27A SYTL5 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3155758 | |
| SYTL5 RAB27A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3080421 | |
| SYTL5 RAB27A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SYTL5 RAB27A | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RAB27A SYTL5 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | 2698812 |
Curated By
- BioGRID