An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Comprehensive Proteomic Analysis of PGC7-Interacting Proteins.

Liu H, Zhang L, Wei Q, Shi Z, Shi X, Du J, Huang C, Zhang Y, Guo Z

Primordial germ cell 7 (PGC7), a maternal factor essential for early development, plays a critical role in the regulation of DNA methylation, transcriptional repression, chromatin condensation, and cell division and the maintenance of cell pluripotentiality. Despite the fundamental roles of PGC7 in these cellular processes, only a few molecular and functional interactions of PGC7 have been reported. Here, a streptavidin-biotin ... [more]

J. Proteome Res. Dec. 01, 2016; 16(9);3113-3123 [Pubmed: 28712289]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

DPPA3

Gene Ontology Biological Process

Gene Ontology Molecular Function

methylated histone binding [IMP]protein binding [IPI]

Gene Ontology Cellular Component

EXD2