BAIT

LHY

LATE ELONGATED HYPOCOTYL, LATE ELONGATED HYPOCOTYL 1, LHY1, T25K16.6, T25K16_6, AT1G01060
protein LHY
Arabidopsis thaliana (Columbia)
PREY

CKB3

casein kinase II beta chain 3, AT3G60250
casein kinase 2 subunit beta-3
GO Process (1)
GO Function (3)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The protein kinase CK2 is involved in regulation of circadian rhythms in Arabidopsis.

Sugano S, Andronis C, Ong MS, Green RM, Tobin EM

A wide range of processes in plants, including expression of certain genes, is regulated by endogenous circadian rhythms. The circadian clock-associated 1 (CCA1) and the late elongated hypocotyl (LHY) proteins have been shown to be closely associated with clock function in Arabidopsis thaliana. The protein kinase CK2 can interact with and phosphorylate CCA1, but its role in the regulation of ... [more]

Proc. Natl. Acad. Sci. U.S.A. Oct. 26, 1999; 96(22);12362-6 [Pubmed: 10535927]

Throughput

  • Low Throughput

Additional Notes

  • Both proteins purified.

Curated By

  • BioGRID