PREY

DDX3X

CAP-Rf, DBX, DDX14, DDX3, HLP2
DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked
GO Process (29)
GO Function (14)
GO Component (6)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identification of a USP9X Substrate NFX1-123 by SILAC-Based Quantitative Proteomics.

Chen X, Lu D, Gao J, Zhu H, Zhou Y, Gao D, Zhou H

The deubiquitinase USP9X is involved in multiple diseases including neurodegeneration, epilepsy, and various types of tumors by targeting different substrates. In the present study, we aimed to explore the potential substrates of USP9X and performed SILAC-based quantitative proteomics to compare these substrates in USP9X-knockdown and wild-type HeLa cells. We consequently carried out Flag-NFX1-123 tag affinity-based mass spectrometry and confirmed that ... [more]

J. Proteome Res. Dec. 07, 2018; 18(6);2654-2665 [Pubmed: 31059266]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DDX3X NFX1
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
2813301

Curated By

  • BioGRID