BAIT

PIF3

F14J9.19, F14J9_19, PAP3, PHOTOCURRENT 1, PHYTOCHROME INTERACTING FACTOR 3, PHYTOCHROME-ASSOCIATED PROTEIN 3, POC1, purple acid phosphatase 3, AT1G09530
transcription factor PIF3
Arabidopsis thaliana (Columbia)
PREY

HFR1

FBI1, LONG HYPOCOTYL IN FAR-RED, REDUCED PHYTOCHROME SIGNALING 1, REDUCED SENSITIVITY TO FAR-RED LIGHT 1, REP1, RSF1, T6A9.4, T6A9_4, AT1G02340
transcription factor HFR1
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

HFR1 encodes an atypical bHLH protein that acts in phytochrome A signal transduction.

Fairchild CD, Schumaker MA, Quail PH

Phytochromes are informational photoreceptors through which plants adapt their growth and development to prevailing light conditions. These adaptations are effected primarily through phytochrome regulation of gene expression by mechanisms that remain unclear. We describe a new mutant, hfr1 (long hypocotyl in far-red), that exhibits a reduction in seedling responsiveness specifically to continuous far-red light (FRc), thereby suggesting a locus likely ... [more]

Genes Dev. Sep. 15, 2000; 14(18);2377-91 [Pubmed: 10995393]

Throughput

  • Low Throughput

Additional Notes

  • Both proteins purified

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HFR1 PIF3
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
268308

Curated By

  • BioGRID