Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The CRY1 blue light photoreceptor of Arabidopsis interacts with phytochrome A in vitro.

Ahmad M, Jarillo JA, Smirnova O, Cashmore AR

Plants have at least two major photosensory receptors: phytochrome (absorbing primarily red/far-red light) and cryptochrome (absorbing blue/UV-A light); considerable physiological and genetic evidence suggests some form of communication or functional dependence between the receptors. Here, we demonstrate in vitro, using purified recombinant photoreceptors, that Arabidopsis CRY1 and CRY2 (cryptochrome) are substrates for phosphorylation by a phytochrome A-associated kinase activity. Several ... [more]

Mol. Cell Jun. 01, 1998; 1(7);939-48 [Pubmed: 9651577]

Throughput

  • Low Throughput

Curated By

  • BioGRID