PHYB
Gene Ontology Biological Process
- abscisic acid metabolic process [IMP]
- chromatin organization [IMP]
- circadian regulation of calcium ion oscillation [IMP]
- entrainment of circadian clock [IMP]
- gravitropism [IMP]
- jasmonic acid mediated signaling pathway [IMP]
- photomorphogenesis [IMP]
- photosynthesis [IMP]
- phototropism [IMP]
- red light signaling pathway [IMP]
- regulation of defense response [IMP]
- regulation of seed germination [IMP]
- regulation of transcription, DNA-templated [ISS]
- response to cold [IMP]
- response to far red light [IMP]
- response to low fluence red light stimulus [IMP]
- stomatal complex development [IMP]
- transpiration [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
NDPK2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
NDPK2 as a signal transducer in the phytochrome-mediated light signaling.
Nucleoside-diphosphate kinase (NDPK) 2 in Arabidopsis has been identified as a phytochrome-interacting protein by using the C-terminal domain of phytochrome A (PhyA) as the bait in yeast two-hybrid screening. The far-red light-absorbing form of phytochrome (Pfr) A stimulates NDPK2 gamma-phosphate exchange activity in vitro. To better understand the multiple functions of NDPK and its role in phytochrome-mediated signaling, we characterized ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PHYB NDPK2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID