BAIT

NIA2

ARABIDOPSIS NITRATE REDUCTASE 2, ATNR2, B29, CHL3, CHLORATE RESISTANT 3, F28L22.2, F28L22_2, NIA2-1, NITRATE REDUCTASE, NITRATE REDUCTASE 2, NR, NR2, AT1G37130
nitrate reductase [NADH] 2
GO Process (4)
GO Function (2)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

GRF3

GF14PSI ISOFORM, MXI10.21, MXI10_21, RCI1, general regulatory factor 3, AT5G38480
14-3-3-like protein GF14 psi
GO Process (0)
GO Function (2)
GO Component (9)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Ser-534 in the hinge 1 region of Arabidopsis nitrate reductase is conditionally required for binding of 14-3-3 proteins and in vitro inhibition.

Kanamaru K, Wang R, Su W, Crawford NM

14-3-3 proteins bind to the hinge 1 region of nitrate reductase (NR) and inhibit its activity. To determine which residues of NR are required for 14-3-3-inhibitory interactions, wild-type and mutant forms of Arabidopsis NR were examined in the yeast two-hybrid system and in vitro inhibition assays. NR fragments with or without hinge 1 were introduced into yeast with one of ... [more]

J. Biol. Chem. Feb. 12, 1999; 274(7);4160-5 [Pubmed: 9933611]

Throughput

  • Low Throughput

Curated By

  • BioGRID