An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

EMBRYONIC FLOWER1 participates in polycomb group-mediated AG gene silencing in Arabidopsis.

Calonje M, Sanchez R, Chen L, Sung ZR

Polycomb group (PcG)-mediated gene silencing is a common developmental strategy used to maintain stably inherited repression of target genes and involves different protein complexes known as Polycomb-repressive complexes (PRCs). In animals, the two best-characterized PcG complexes are PRC1 and PRC2. In this report, we demonstrate that the plant-specific protein EMBRYONIC FLOWER1 (EMF1) functions in maintaining the repression of the flower ... [more]

Plant Cell Feb. 01, 2008; 20(2);277-91 [Pubmed: 18281509]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

MSI1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

EMF1