BAIT

TNFSF11

CD254, ODF, OPGL, OPTB2, RANKL, TRANCE, hRANKL2, sOdf, RP11-86N24.2
tumor necrosis factor (ligand) superfamily, member 11
Homo sapiens
PREY

SRC

ASV, SRC1, c-SRC, p60-Src, RP5-823N20.1
SRC proto-oncogene, non-receptor tyrosine kinase
GO Process (50)
GO Function (17)
GO Component (10)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

TRANCE, a TNF family member, activates Akt/PKB through a signaling complex involving TRAF6 and c-Src.

Wong BR, Besser D, Kim N, Arron JR, Vologodskaia M, Hanafusa H, Choi Y

TRANCE, a TNF family member, and its receptor, TRANCE-R, are critical regulators of dendritic cell and osteoclast function. Here, we demonstrate that TRANCE activates the antiapoptotic serine/threonine kinase Akt/PKB through a signaling complex involving c-Src and TRAF6. A deficiency in c-Src or addition of Src family kinase inhibitors blocks TRANCE-mediated PKB activation in osteoclasts. c-Src and TRAF6 interact with each ... [more]

Mol. Cell Dec. 01, 1999; 4(6);1041-9 [Pubmed: 10635328]

Throughput

  • Low Throughput

Curated By

  • BioGRID