An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Intrinsically Disordered Protein TEX264 Mediates ER-phagy.

Chino H, Hatta T, Natsume T, Mizushima N

Certain proteins and organelles can be selectively degraded by autophagy. Typical substrates and receptors of selective autophagy have LC3-interacting regions (LIRs) that bind to autophagosomal LC3 and GABARAP family proteins. Here, we performed a differential interactome screen using wild-type LC3B and a LIR recognition-deficient mutant and identified TEX264 as a receptor for autophagic degradation of the endoplasmic reticulum (ER-phagy). TEX264 ... [more]

Mol. Cell Dec. 06, 2018; 74(5);909-921.e6 [Pubmed: 31006538]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

MAP1LC3B

Gene Ontology Biological Process

Gene Ontology Molecular Function

GABA receptor binding [IBA]microtubule binding [IBA]protein binding [IPI]

Gene Ontology Cellular Component

LMO7