SE
Gene Ontology Biological Process
- RNA splicing [IMP]
- chromatin modification [TAS]
- primary miRNA processing [IMP]
- production of ta-siRNAs involved in RNA interference [IMP]
- regulation of adaxial/abaxial pattern formation [IMP]
- regulation of meristem development [IMP]
- regulation of transcription, DNA-templated [TAS]
- shoot system development [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
DCL1
Gene Ontology Biological Process
- RNA processing [ISS]
- cytokinesis by cell plate formation [IMP]
- embryonic pattern specification [IMP]
- flower development [TAS]
- mRNA cleavage involved in gene silencing by miRNA [IMP]
- primary miRNA processing [TAS]
- production of lsiRNA involved in RNA interference [IMP]
- production of miRNAs involved in gene silencing by miRNA [IGI]
- production of siRNA involved in RNA interference [IMP]
- production of ta-siRNAs involved in RNA interference [IMP]
- regulation of seed maturation [IMP]
- seed morphogenesis [IMP]
- suspensor development [IMP]
- vegetative to reproductive phase transition of meristem [IMP]
- virus induced gene silencing [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
Identification of nuclear dicing bodies containing proteins for microRNA biogenesis in living Arabidopsis plants.
MicroRNAs (miRNAs) are important for regulating gene expression in muticellular organisms. MiRNA processing is a two-step process. In animal cells, the first step is nuclear and the second step cytoplasmic, whereas in plant cells, both steps occur in the nucleus via the enzyme Dicer-like1 (DCL1) and other proteins including the zinc-finger-domain protein Serrate (SE) and a double-stranded RNA (dsRNA) binding-domain ... [more]
Throughput
- Low Throughput
Additional Notes
- BiFC
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DCL1 SE | PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay. | Low | - | BioGRID | - | |
| SE DCL1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID