AGO1
Gene Ontology Biological Process
- RNA interference [IMP]
- adaxial/abaxial pattern specification [IMP]
- adventitious root development [IMP]
- auxin metabolic process [IGI]
- embryo development ending in seed dormancy [IGI]
- gene silencing by miRNA [IEP, IGI, IMP]
- innate immune response [IMP]
- leaf morphogenesis [IMP]
- leaf proximal/distal pattern formation [IMP]
- leaf vascular tissue pattern formation [IMP]
- posttranscriptional gene silencing [IEP]
- response to auxin [IGI]
- response to far red light [IGI]
- stem cell development [IGI]
- virus induced gene silencing [IDA]
Gene Ontology Molecular Function
HYL1
Gene Ontology Biological Process
- leaf proximal/distal pattern formation [IMP]
- leaf vascular tissue pattern formation [IMP]
- mRNA cleavage involved in gene silencing by miRNA [IMP]
- pre-miRNA processing [IMP]
- production of miRNAs involved in gene silencing by miRNA [IMP]
- production of ta-siRNAs involved in RNA interference [IMP]
- response to abscisic acid [IMP]
- response to auxin [IMP]
- response to cytokinin [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
Identification of nuclear dicing bodies containing proteins for microRNA biogenesis in living Arabidopsis plants.
MicroRNAs (miRNAs) are important for regulating gene expression in muticellular organisms. MiRNA processing is a two-step process. In animal cells, the first step is nuclear and the second step cytoplasmic, whereas in plant cells, both steps occur in the nucleus via the enzyme Dicer-like1 (DCL1) and other proteins including the zinc-finger-domain protein Serrate (SE) and a double-stranded RNA (dsRNA) binding-domain ... [more]
Throughput
- Low Throughput
Additional Notes
- BiFC
Curated By
- BioGRID