BAIT

OASA1

ATCYS-3A, CYTACS1, DL3480C, FCAALL.34, O-ACETHYLSERINE SULFHYDRYLASE, O-ACETYLSERINE (THIOL)LYASE, O-acetylserine (thiol) lyase (OAS-TL) isoform A1, OLD3, ONSET OF LEAF DEATH 3, AT4G14880
O-acetylserine (thiol) lyase (OAS-TL) isoform A1
Arabidopsis thaliana (Columbia)
PREY

SERAT2;1

ATSERAT2;1, F14J16.18, F14J16_18, SAT1, SAT5, SERINE ACETYLTRANSFERASE, SERINE ACETYLTRANSFERASE 1, SERINE ACETYLTRANSFERASE 5, serine acetyltransferase 2;1, AT1G55920
serine acetyltransferase 1
GO Process (2)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Thermodynamics of the interaction between O-acetylserine sulfhydrylase and the C-terminus of serine acetyltransferase.

Kumaran S, Jez JM

Cysteine biosynthesis in plants is partly regulated by the physical association of O-acetylserine sulfhydrylase (OASS) and serine acetyltransferase (SAT). Interaction of OASS and SAT requires only the 10 C-terminal residues of SAT. Here we analyze the thermodynamics of formation of a complex of Arabidopsis thaliana OASS (AtOASS) and the C-terminal ligand of AtSAT (C10 peptide) as a function of temperature ... [more]

Biochemistry May. 08, 2007; 46(18);5586-94 [Pubmed: 17425333]

Throughput

  • Low Throughput

Additional Notes

  • sat peptide

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
OASA1 SERAT2;1
Co-crystal Structure
Co-crystal Structure

Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.

Low-BioGRID
-

Curated By

  • BioGRID