Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Trafficking of the human transferrin receptor in plant cells: effects of tyrphostin A23 and brefeldin A.

Ortiz-Zapater E, Soriano-Ortega E, Marcote MJ, Ortiz-Masia D, Aniento F

Plant cells possess much of the molecular machinery necessary for receptor-mediated endocytosis (RME), but this process still awaits detailed characterization. In order to identify a reliable and well-characterized marker to investigate RME in plant cells, we have expressed the human transferrin receptor (hTfR) in Arabidopsis protoplasts. We have found that hTfR is mainly found in endosomal (Ara7- and FM4-64-positive) compartments, ... [more]

Plant J. Dec. 01, 2006; 48(5);757-70 [Pubmed: 17059402]

Throughput

  • Low Throughput

Curated By

  • BioGRID