BAIT

TOC64-III

AT3G17960, atToc64-III, translocon at the outer membrane of chloroplasts 64-III, AT3G17970
translocon at the outer membrane of chloroplasts 64-III
GO Process (0)
GO Function (0)
GO Component (4)

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

AKR2

AFT, AKR2A, F15J1.20, F15J1_20, ankyrin repeat-containing protein 2, AT4G35450
ankyrin repeat domain-containing protein 2
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

AKR2A-mediated import of chloroplast outer membrane proteins is essential for chloroplast biogenesis.

Bae W, Lee YJ, Kim DH, Lee J, Kim S, Sohn EJ, Hwang I

In plant cells, chloroplasts have essential roles in many biochemical reactions and physiological responses. Chloroplasts require numerous protein components, but only a fraction of these proteins are encoded by the chloroplast genome. Instead, most are encoded by the nuclear genome and imported into chloroplasts from the cytoplasm post-translationally. Membrane proteins located in the chloroplast outer envelope membrane (OEM) have a ... [more]

Nat. Cell Biol. Feb. 01, 2008; 10(2);220-7 [Pubmed: 18193034]

Throughput

  • Low Throughput

Curated By

  • BioGRID