BAIT

TOC33

ATTOC33, PLASTID PROTEIN IMPORT 1, PPI1, T6A9.32, translocon at the outer envelope membrane of chloroplasts 33, AT1G02280
translocase of chloroplast 33
GO Process (1)
GO Function (5)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

AKR2

AFT, AKR2A, F15J1.20, F15J1_20, ankyrin repeat-containing protein 2, AT4G35450
ankyrin repeat domain-containing protein 2
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

AKR2A-mediated import of chloroplast outer membrane proteins is essential for chloroplast biogenesis.

Bae W, Lee YJ, Kim DH, Lee J, Kim S, Sohn EJ, Hwang I

In plant cells, chloroplasts have essential roles in many biochemical reactions and physiological responses. Chloroplasts require numerous protein components, but only a fraction of these proteins are encoded by the chloroplast genome. Instead, most are encoded by the nuclear genome and imported into chloroplasts from the cytoplasm post-translationally. Membrane proteins located in the chloroplast outer envelope membrane (OEM) have a ... [more]

Nat. Cell Biol. Feb. 01, 2008; 10(2);220-7 [Pubmed: 18193034]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
AKR2 TOC33
Co-localization
Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Low-BioGRID
-

Curated By

  • BioGRID