SNAREs and Rab GTPases cooperate in vesicle transport through a mechanism yet poorly understood. We now demonstrate that the Rab5 effectors EEA1 and Rabaptin-5/Rabex-5 exist on the membrane in high molecular weight oligomers, which also contain NSF. Oligomeric assembly is modulated by the ATPase activity of NSF. Syntaxin 13, the t-SNARE required for endosome fusion, is transiently incorporated into the ... [more]

Cell Aug. 06, 1999; 98(3);377-86 [Pubmed: 10458612]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

EEA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

1-phosphatidylinositol binding [IDA]GTP-dependent protein binding [IDA]calmodulin binding [NAS]protein binding [IPI]protein homodimerization activity [IDA]zinc ion binding [TAS]

Gene Ontology Cellular Component

STX12

Gene Ontology Biological Process

Gene Ontology Molecular Function

SNAP receptor activity [IBA]SNARE binding [IBA]protein binding [IPI]

Gene Ontology Cellular Component

Reconstituted Complex

Publication

Oligomeric complexes link Rab5 effectors with NSF and drive membrane fusion via interactions between EEA1 and syntaxin 13.

Throughput

Curated By

1-phosphatidylinositol binding [IDA]
GTP-dependent protein binding [IDA]
calmodulin binding [NAS]
protein binding [IPI]
protein homodimerization activity [IDA]
zinc ion binding [TAS]

SNAP receptor activity [IBA]
SNARE binding [IBA]
protein binding [IPI]