BAIT

MYA2

ARABIDOPSIS MYOSIN 2, ATMYA2, F6B6.4, F6B6_4, MYOSIN X1 2, MYOSIN XI-6, XI-2, XI-6, myosin 2, AT5G43900
myosin 2
Arabidopsis thaliana (Columbia)
PREY

ATFP8

ARABIDOPSIS THALIANA RAB GTPASE HOMOLOG D1, ATRABD1, RAB GTPASE HOMOLOG D1, RABD1, AT3G11730
Ras-related protein RABD1
GO Process (1)
GO Function (4)
GO Component (4)
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

An isoform of Arabidopsis myosin XI interacts with small GTPases in its C-terminal tail region.

Hashimoto K, Igarashi H, Mano S, Takenaka C, Shiina T, Yamaguchi M, Demura T, Nishimura M, Shimmen T, Yokota E

Myosin XI, a class of myosins expressed in plants is believed to be responsible for cytoplasmic streaming and the translocation of organelles and vesicles. To gain further insight into the translocation of organelles and vesicles by myosin XI, an isoform of Arabidopsis myosin XI, MYA2, was chosen and its role in peroxisome targeting was examined. Using the yeast two-hybrid screening ... [more]

J. Exp. Bot. Aug. 16, 2008; 59(13);3523-31 [Pubmed: 18703495]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ATFP8 MYA2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID