Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Toward an interaction map of the two-component signaling pathway of Arabidopsis thaliana.

Dortay H, Gruhn N, Pfeifer A, Schwerdtner M, Schmuelling T, Heyl A

Among the signal transduction pathways in higher eukaryotes, the two-component system (TCS) is unique to plants. In the model plant Arabidopsis thaliana, it consists of more than 30 proteins, including eight receptors, five phosphotransmitters and 23 response regulators. One of its important functions is to perceive and transduce the signal of the plant hormone cytokinin. The basic signal flow within ... [more]

J. Proteome Res. Sep. 01, 2008; 7(9);3649-60 [Pubmed: 18642946]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

RR14

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]phosphorelay response regulator activity [ISS]protein binding [IPI]sequence-specific DNA binding transcription factor activity [ISS]

Gene Ontology Cellular Component

HSP17.4