Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Rab coupling protein (RCP), a novel Rab4 and Rab11 effector protein.

Lindsay AJ, Hendrick AG, Cantalupo G, Senic-Matuglia F, Goud B, Bucci C, McCaffrey MW

Rab4 and Rab11 are small GTPases belonging to the Ras superfamily. They both function as regulators along the receptor recycling pathway. We have identified a novel 80-kDa protein that interacts specifically with the GTP-bound conformation of Rab4, and subsequent work has shown that it also interacts strongly with Rab11. We name this protein Rab coupling protein (RCP). RCP is predominantly ... [more]

J. Biol. Chem. Apr. 05, 2002; 277(14);12190-9 [Pubmed: 11786538]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RAB4A RAB11FIP1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Go CD (2021)	High	6.54	BioGRID	3002583
RAB4A RAB11FIP1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Lindsay AJ (2002)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

RAB4A

Gene Ontology Biological Process

Gene Ontology Molecular Function

GDP binding [IDA]GTP binding [IDA]GTPase activity [IDA]protein binding [IPI]

Gene Ontology Cellular Component

RAB11FIP1