BAIT

ICK1

CYCLIN-DEPENDENT KINASE INHIBITOR PROTEIN, F26B6.8, F26B6_8, KIP-RELATED PROTEIN 1, KRP1, AT2G23430
cyclin-dependent kinase inhibitor 1
Arabidopsis thaliana (Columbia)
PREY

RKP

AT2G22020, F7D8.33, F7D8_33, related to KPC1, AT2G22010
E3 ubiquitin-protein ligase RKP
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

RKP, a RING finger E3 ligase induced by BSCTV C4 protein, affects geminivirus infection by regulation of the plant cell cycle.

Lai J, Chen H, Teng K, Zhao Q, Zhang Z, Li Y, Liang L, Xia R, Wu Y, Guo H, Xie Q

The C4 protein from Curtovirus is known as a major symptom determinant, but the mode of action of the C4 protein remains unclear. To understand the mechanism of involvement of C4 protein in virus-plant interactions, we introduced the C4 gene from Beet severe curly top virus (BSCTV) into Arabidopsis under a conditional expression promoter; the resulting overexpression of BSCTV C4 ... [more]

Plant J. Mar. 01, 2009; 57(5);905-17 [Pubmed: 19000158]

Throughput

  • Low Throughput

Curated By

  • BioGRID