BAIT

AT2G39630

F12L6.29, F12L6_29
putative dolichyl-phosphate beta-glucosyltransferase
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

ALB3

ABL3, ALBINO 3, CHLOROPLAST MEMBRANE PROTEIN ALB3, F8N16.9, F8N16_9, AT2G28800
inner membrane protein ALBINO3
Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

The yeast split-ubiquitin system to study chloroplast membrane protein interactions.

Pasch JC, Nickelsen J, Schuenemann D

Each photosynthetic complex within the thylakoid membrane consists of several different subunits. During formation of these complexes, numerous regulatory factors are required for the coordinated transport and assembly of the subunits. Interactions between transport/assembly factors and their specific polypeptides occur in a membraneous environment and are usually transient and short-lived. Thus, a detailed analysis of the underlying molecular mechanisms by ... [more]

Appl. Microbiol. Biotechnol. Dec. 01, 2005; 69(4);440-7 [Pubmed: 15988575]

Throughput

  • Low Throughput

Additional Notes

  • split-ubiquitin

Curated By

  • BioGRID