BAIT

FH5

ATFH5, FORMIN HOMOLOGY 2 DOMAIN-CONTAINING PROTEIN 5, FORMIN HOMOLOGY 5, MRB17.15, MRB17_15, formin homology5, AT5G54650
formin-like protein 5
GO Process (4)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

ACT12

ACTIN, actin-12, AT3G46520
actin-12
GO Process (1)
GO Function (1)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Plant formin AtFH5 is an evolutionarily conserved actin nucleator involved in cytokinesis.

Ingouff M, Fitz Gerald JN, Guerin C, Robert H, Sorensen MB, Van Damme D, Geelen D, Blanchoin L, Berger F

Formins are actin-organizing proteins that are involved in cytokinesis and cell polarity. In the plant Arabidopsis thaliana, there are more than 20 formin homologues, all of which have unknown roles. In this study, we characterize specific cellular and molecular functions of the Arabidopsis formin AtFH5. Despite the low identity of AtFH5 to yeast and mammalian formins, the AtFH5 protein interacts ... [more]

Nat. Cell Biol. Apr. 01, 2005; 7(4);374-80 [Pubmed: 15765105]

Throughput

  • Low Throughput

Curated By

  • BioGRID