An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

A receptor-like kinase from Arabidopsis thaliana is a calmodulin-binding protein.

Charpenteau M, Jaworski K, Ramirez BC, Tretyn A, Ranjeva R, Ranty B

Screening a cDNA expression library with a radiolabelled calmodulin (CaM) probe led to the isolation of AtCaMRLK, a receptor-like kinase (RLK) of Arabidopsis thaliana. AtCaMRLK polypeptide sequence shows a modular organization consisting of the four distinctive domains characteristic of receptor kinases: an amino terminal signal sequence, a domain containing seven leucine-rich repeats, a single putative membrane-spanning segment and a protein ... [more]

Biochem. J. May. 01, 2004; 379(0);841-8 [Pubmed: 14720124]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

CRCK1

Gene Ontology Biological Process

Gene Ontology Molecular Function

calmodulin binding [IDA]calmodulin-dependent protein kinase activity [IDA]kinase activity [ISS]protein serine/threonine kinase activity [IDA]

Gene Ontology Cellular Component

CAM1