BAIT

MBD5

ATMBD5, MBD05, MDB05, METHYL-CPG-BINDING DOMAIN PROTEIN 05, methyl-CPG-binding domain protein 5, AT3G46580
methyl-CPG-binding domain protein 5
GO Process (0)
GO Function (5)
GO Component (3)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

CHR1

ATDDM1, CHA1, CHR01, CHROMATIN REMODELING 1, DDM1, DECREASED DNA METHYLATION 1, MSN2.14, MSN2_14, SOM1, SOM4, SOMNIFEROUS 1, AT5G66750
ATP-dependent DNA helicase DDM1
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

DDM1 binds Arabidopsis methyl-CpG binding domain proteins and affects their subnuclear localization.

Zemach A, Li Y, Wayburn B, Ben-Meir H, Kiss V, Avivi Y, Kalchenko V, Jacobsen SE, Grafi G

Methyl-CpG binding domain (MBD) proteins in Arabidopsis thaliana bind in vitro methylated CpG sites. Here, we aimed to characterize the binding properties of AtMBDs to chromatin in Arabidopsis nuclei. By expressing in wild-type cells AtMBDs fused to green fluorescent protein (GFP), we showed that AtMBD7 was evenly distributed at all chromocenters, whereas AtMBD5 and 6 showed preference for two perinucleolar ... [more]

Plant Cell May. 01, 2005; 17(5);1549-58 [Pubmed: 15805479]

Throughput

  • Low Throughput

Curated By

  • BioGRID