BAIT

ABI5

ABA INSENSITIVE 5, F2H17.12, F2H17_12, GIA1, GROWTH-INSENSITIVITY TO ABA 1, AT2G36270
protein abscisic acid-insensitive 5
Arabidopsis thaliana (Columbia)
PREY

ACS10

ACC synthase 10, F16P17.11, F16P17_11, AT1G62960
putative aminotransferase ACS10
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

AFP is a novel negative regulator of ABA signaling that promotes ABI5 protein degradation.

Lopez-Molina L, Mongrand S, Kinoshita N, Chua NH

Plants have evolved protective mechanisms to ensure their survival when threatened by adverse environmental conditions during their transition to autotrophic growth. During germination, there is a 2- to 3-d period during which a plant can execute growth arrest when challenged by water deficit. This postgermination developmental checkpoint is signaled by the stress hormone abscisic acid (ABA), which induces the expression ... [more]

Genes Dev. Feb. 01, 2003; 17(3);410-8 [Pubmed: 12569131]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ACS10 ABI5
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
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Curated By

  • BioGRID