BAIT

CLF

CURLY LEAF, F26B6.3, F26B6_3, ICU1, INCURVATA 1, SDG1, SET1, SETDOMAIN 1, SETDOMAIN GROUP 1, AT2G23380
histone-lysine N-methyltransferase CLF
Arabidopsis thaliana (Columbia)
PREY

EMF2

AT5G51240, AtEMF2, CYR1, CYTOKININ RESISTANT 1, EMBRYONIC FLOWER 2, MWD22.18, MWD22_18, VEF2, AT5G51230
polycomb group protein EMBRYONIC FLOWER 2
GO Process (2)
GO Function (3)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Interaction of Polycomb-group proteins controlling flowering in Arabidopsis.

Chanvivattana Y, Bishopp A, Schubert D, Stock C, Moon YH, Sung ZR, Goodrich J

In Arabidopsis, the EMBYRONIC FLOWER2 (EMF2), VERNALISATION2 (VRN2) and FERTILISATION INDEPENDENT ENDOSPERM2 (FIS2) genes encode related Polycomb-group (Pc-G) proteins. Their homologues in animals act together with other Pc-G proteins as part of a multimeric complex, Polycomb Repressive Complex 2 (PRC2), which functions as a histone methyltransferase. Despite similarities between the fis2 mutant phenotype and those of some other plant Pc-G ... [more]

Development Nov. 01, 2004; 131(21);5263-76 [Pubmed: 15456723]

Throughput

  • Low Throughput

Additional Notes

  • split ubiquitin

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CLF EMF2
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
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Curated By

  • BioGRID