BAIT

ATB' BETA

AT3G09880
serine/threonine protein phosphatase 2A regulatory subunit B prime beta isoform
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

RCN1

ATB BETA BETA, EER1, ENHANCED ETHYLENE RESPONSE 1, F2J7.19, F2J7_19, PHOSPHOPROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT A, REGA, ROOTS CURL IN NPA, SERINE/THREONINE PROTEIN PHOSPHATASE TYPE 2A REGULATORY SUBUNIT A, AT1G25490
serine/threonine-protein phosphatase 2A regulatory subunit A alpha isoform
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Molecular characterization of the B' regulatory subunit gene family of Arabidopsis protein phosphatase 2A.

Haynes JG, Hartung AJ, Hendershot JD, Passingham RS, Rundle SJ

Type 2A serine/threonine protein phosphatases (PP2A) have been implicated as important mediators of a diverse array of reversible protein phosphorylation events in plants. We have identified a novel Arabidopsis gene (AtB' delta) which encodes a 55-kDa B' type regulatory subunit of PP2A. The protein encoded by this gene is 57-63% identical and 69-74% similar to the previously identified AtB' genes. ... [more]

Eur. J. Biochem. Feb. 01, 1999; 260(1);127-36 [Pubmed: 10091592]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RCN1 ATB' BETA
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
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Curated By

  • BioGRID