An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

An SC35-like protein and a novel serine/arginine-rich protein interact with Arabidopsis U1-70K protein.

Golovkin M, Reddy AS

The U1 small nuclear ribonucleoprotein 70-kDa protein, a U1 small nuclear ribonucleoprotein-specific protein, has been shown to have multiple roles in nuclear precursor mRNA processing in animals. By using the C-terminal arginine-rich region of Arabidopsis U1-70K protein in the yeast two-hybrid system, we have identified an SC35-like (SR33) and a novel plant serine/arginine-rich (SR) protein (SR45) that interact with the ... [more]

J. Biol. Chem. Dec. 17, 1999; 274(51);36428-38 [Pubmed: 10593939]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

FC2

Gene Ontology Biological Process

Gene Ontology Molecular Function

kinase activity [ISS]protein binding [IPI]protein kinase activity [IDA]protein serine/threonine kinase activity [IDA]

Gene Ontology Cellular Component

SRZ-22