CIP8
Gene Ontology Biological Process
Gene Ontology Molecular Function
HY5
Gene Ontology Biological Process
- gibberellic acid mediated signaling pathway [IMP]
- photomorphogenesis [TAS]
- positive regulation of anthocyanin metabolic process [IMP]
- positive regulation of circadian rhythm [IMP]
- red or far-red light signaling pathway [IMP]
- regulation of photomorphogenesis [IMP]
- regulation of transcription, DNA-templated [ISS, TAS]
- response to UV-B [IEP, IGI, IMP]
- response to abscisic acid [IMP]
- response to far red light [IEP]
- response to karrikin [IEP]
- response to red light [IEP]
Gene Ontology Molecular Function
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Biochemical evidence for ubiquitin ligase activity of the Arabidopsis COP1 interacting protein 8 (CIP8).
Arabidopsis COP1 is a negative regulator of photomorphogenesis, which targets HY5, a positive regulator of photomorphogenesis, for degradation via the proteasome pathway in the absence of light. COP1 and its interactive partner CIP8 both possess RING finger motifs, characteristic of some E3 ubiquitin ligases. Here we show that CIP8 promotes ubiquitin attachment to HY5 in E2-dependent fashion in vitro. CIP8 ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID