BAIT

RBX1

ATRBX1, F7C8.160, F7C8_160, HRT1, REGULATOR OF CULLINS-1, RING-BOX 1, ROC1, AT5G20570
RING-box protein 1
GO Process (2)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

TIR

F3N23.13, F3N23_13, toll/interleukin-1 receptor-like, AT1G72930
toll/interleukin-1 receptor-like protein
GO Process (1)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Role of the Arabidopsis RING-H2 protein RBX1 in RUB modification and SCF function.

Gray WM, Hellmann H, Dharmasiri S, Estelle M

The ubiquitin-related protein RUB/Nedd8 is conjugated to members of the cullin family of proteins in plants, animals, and fungi. In Arabidopsis, the RUB conjugation pathway consists of a heterodimeric E1 (AXR1-ECR1) and a RUB-E2 called RCE1. The cullin CUL1 is a subunit in SCF-type ubiquitin protein ligases (E3s), including the SCF(TIR1) complex, which is required for response to the plant ... [more]

Plant Cell Sep. 01, 2002; 14(9);2137-44 [Pubmed: 12215511]

Throughput

  • Low Throughput

Curated By

  • BioGRID