BAIT

RCE1

AP22.40, AP22_40, RUB1 conjugating enzyme 1, AT4G36800
RUB1 conjugating enzyme 1
GO Process (2)
GO Function (3)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Arabidopsis thaliana (Columbia)
PREY

GRH1

A. THALIANA GERMIN-LIKE PROTEIN 1, AFB1, ATFBL18, ATGER1, ATGRH1, AUXIN SIGNALING F BOX PROTEIN 1, F4C21.11, F4C21_11, GRR1-like protein 1, AT4G03190
GRR1-like protein 1
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The RUB/Nedd8 conjugation pathway is required for early development in Arabidopsis.

Dharmasiri S, Dharmasiri N, Hellmann H, Estelle M

The related-to-ubiquitin (RUB) protein is post-translationally conjugated to the cullin subunit of the SCF (SKP1, Cullin, F-box) class of ubiquitin protein ligases. Although the precise biochemical function of RUB modification is unclear, studies indicate that the modification is important for SCF function. In Arabidopsis, RUB modification of CUL1 is required for normal function of SCF(TIR1), an E3 required for response ... [more]

EMBO J. Apr. 15, 2003; 22(8);1762-70 [Pubmed: 12682009]

Throughput

  • Low Throughput

Curated By

  • BioGRID