BAIT

SOS2

ATSOS2, CBL-INTERACTING PROTEIN KINASE 24, CIPK24, K21B8.3, K21B8_3, SALT OVERLY SENSITIVE 2, SNF1-RELATED PROTEIN KINASE 3.11, SNRK3.11, AT5G35410
CBL-interacting serine/threonine-protein kinase 24
GO Process (1)
GO Function (4)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

ABI2

ABA INSENSITIVE 2, AtABI2, MHM17.19, MHM17_19, PROTEIN PHOSPHATASE 2C, AT5G57050
protein phosphatase 2C 77
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A novel domain in the protein kinase SOS2 mediates interaction with the protein phosphatase 2C ABI2.

Ohta M, Guo Y, Halfter U, Zhu JK

SOS2 (salt overly sensitive 2) is a serine/threonine protein kinase required for salt tolerance in Arabidopsis thaliana. In this study, we identified the protein phosphatase 2C ABI2 (abscisic acid-insensitive 2) as a SOS2-interacting protein. Deletion analysis led to the discovery of a novel protein domain of 37 amino acid residues, designated as the protein phosphatase interaction (PPI) motif, of SOS2 ... [more]

Proc. Natl. Acad. Sci. U.S.A. Sep. 30, 2003; 100(20);11771-6 [Pubmed: 14504388]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ABI2 SOS2
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
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Curated By

  • BioGRID