BAIT

TT8

BHLH42, F17A8.170, F17A8_170, TRANSPARENT TESTA 8, AT4G09820
transcription factor TT8
Arabidopsis thaliana (Columbia)
PREY

MYB114

AtMYB114, T27F4.13, T27F4_13, myb domain protein 114, AT1G66380
transcription factor MYB114
GO Process (3)
GO Function (2)
GO Component (1)
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Comprehensive identification of Arabidopsis thaliana MYB transcription factors interacting with R/B-like BHLH proteins.

Zimmermann IM, Heim MA, Weisshaar B, Uhrig JF

In-depth analysis of protein-protein interaction specificities of the MYB protein family of Arabidopsis thaliana revealed a conserved amino acid signature ([DE]Lx(2)[RK]x(3)Lx(6)Lx(3)R) as the structural basis for interaction between MYB and R/B-like BHLH proteins. The motif has successfully been used to predict new MYB/BHLH interactions for A. thaliana proteins, it allows to discriminate between even closely related MYB proteins and it ... [more]

Plant J. Oct. 01, 2004; 40(1);22-34 [Pubmed: 15361138]

Throughput

  • Low Throughput

Curated By

  • BioGRID