Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Molecular and functional characterization of Arabidopsis Cullin 3A.

Dieterle M, Thomann A, Renou JP, Parmentier Y, Cognat V, Lemonnier G, Mueller R, Shen WH, Kretsch T, Genschik P

Cullin proteins, which belong to multigenic families in all eukaryotes, associate with other proteins to form ubiquitin protein ligases (E3s) that target substrates for proteolysis by the 26S proteasome. Here, we present the molecular and genetic characterization of a plant Cullin3. In contrast to fungi and animals, the genome of the model plant Arabidopsis thaliana contains two related CUL3 genes, ... [more]

Plant J. Feb. 01, 2005; 41(3);386-99 [Pubmed: 15659098]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

CUL3

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]ubiquitin-protein transferase activity [ISS]

Gene Ontology Cellular Component

ABAP1