BAIT

AG

AGAMOUS, AGAMOUS PROTEIN, F13C5.130, F13C5_130, AT4G18960
MADS domain transcription factor AGAMOUS
GO Process (4)
GO Function (3)
GO Component (1)
Arabidopsis thaliana (Columbia)
PREY

AGL6

AGAMOUS-like 6, F17K2.18, AT2G45650
agamous-like MADS-box protein AGL6
GO Process (3)
GO Function (3)
GO Component (1)
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Specific interactions between the K domains of AG and AGLs, members of the MADS domain family of DNA binding proteins.

Fan HY, Hu Y, Tudor M, Ma H

MADS domain (for MCM1, AG, DEFA and SRF) proteins are regulatory proteins found in all major eukaryotic kingdoms. Plant MADS domain regulatory proteins have a region of moderate sequence similarity that has been designated as the K domain, and its predicted coiled-coil structure suggests a role in establishing a protein-protein interaction. In vivo studies with the Arabidopsis AGAMOUS (AG) protein ... [more]

Plant J. Nov. 01, 1997; 12(5);999-1010 [Pubmed: 9418042]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
AG AGL6
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID