BAIT

KAT1

MPL12.2, MPL12_2, potassium channel in Arabidopsis thaliana 1, AT5G46240
potassium channel KAT1
GO Process (1)
GO Function (3)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

KAB1

K+ CHANNEL BETA SUBUNIT PROTEIN, KV-BETA1, POTASSIUM CHANNEL BETA SUBUNIT HOMOLOG, T1G11.6, T1G11_6, potassium channel beta subunit 1, AT1G04690
potassium channel beta subunit 1
GO Process (1)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Physical association of KAB1 with plant K+ channel alpha subunits.

Tang H, Vasconcelos AC, Berkowitz GA

K+ channel proteins contain four alpha subunits that align along a central axis perpendicular to membranes and form an ion-conducting pore. Recent work with K+ channels native to animal membranes has shown that at least some members of this protein family also have four beta subunits. These structural components of the holoenzyme each form tight associations with the cytoplasmic portion ... [more]

Plant Cell Sep. 01, 1996; 8(9);1545-53 [Pubmed: 8837508]

Throughput

  • Low Throughput

Curated By

  • BioGRID