An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Characterization of the interaction between the plasma membrane H-ATPase of Arabidopsis thaliana and a novel interactor (PPI1).

Viotti C, Luoni L, Morandini P, De Michelis MI

Proton pump interactor, isoform 1 (PPI1) is a novel interactor of the C-terminus of Arabidopsis thaliana plasma membrane H(+)-ATPase (EC 3.6.3.6). We produced two fusion proteins consisting of, respectively, the first 88 amino acids or the entire protein deleted of the last 24 hydrophobic amino acids, and we show that the latter protein has a threefold higher affinity for the ... [more]

FEBS J. Nov. 01, 2005; 272(22);5864-71 [Pubmed: 16279950]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

HA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activity [IMP, ISS]hydrogen-exporting ATPase activity, phosphorylative mechanism [IDA]magnesium ion binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

PPI1