BAIT

BRCA2(IV)

BRCA2A, BREAST CANCER 2 like 2A, EDA20, EMBRYO SAC DEVELOPMENT ARREST 20, F6N15.14, F6N15_14, MATERNAL EFFECT EMBRYO ARREST 43, MEE43, AT4G00020
breast cancer protein 2 like 2A
GO Process (5)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

DSS1(I)

ATDSS1(I), F13O11.6, F13O11_6, deletion of SUV3 suppressor 1(I), AT1G64750
probable 26S proteasome complex subunit sem1-1
GO Process (0)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Interaction between Arabidopsis Brca2 and its partners Rad51, Dmc1, and Dss1.

Dray E, Siaud N, Dubois E, Doutriaux MP

The Arabidopsis (Arabidopsis thaliana) orthologs of Brca2, a protein whose mutations are involved in breast cancer in humans, were previously shown to be essential at meiosis. In an attempt to better understand the Brca2-interacting properties, we examined four partners of the two isoforms of Brca2 identified in Arabidopsis (AtRad51, AtDmc1, and two AtDss1 isoforms). The two Brca2 and the two ... [more]

Plant Physiol. Mar. 01, 2006; 140(3);1059-69 [Pubmed: 16415210]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BRCA2(IV) DSS1(I)
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
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Curated By

  • BioGRID