BAIT
BZO2H1
ATBZIP10, Arabidopsis thaliana basic leucine zipper 10, AT4G02640
basic leucine zipper 10
GO Process (4)
GO Function (5)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Arabidopsis thaliana (Columbia)
PREY
BZIP44
AtbZIP44, F1B16.8, F1B16_8, basic leucine-zipper 44, AT1G75390
basic leucine-zipper 44
GO Process (2)
GO Function (6)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Arabidopsis thaliana (Columbia)
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Two-hybrid protein-protein interaction analysis in Arabidopsis protoplasts: establishment of a heterodimerization map of group C and group S bZIP transcription factors.
In vivo protein-protein interactions are frequently studied by means of yeast two-hybrid analysis. However, interactions detected in yeast might differ considerably in the plant system. Based on GAL4 DNA-binding (BD) and activation domains (AD) we established an Arabidopsis protoplast two-hybrid (P2H) system. The use of Gateway-compatible vectors enables the high-throughput screening of protein-protein interactions in plant cells. The efficiency of ... [more]
Plant J. Jun. 01, 2006; 46(5);890-900 [Pubmed: 16709202]
Throughput
- Low Throughput
Curated By
- BioGRID