BAIT

BZIP2

ATBZIP2, F8D23.6, F8D23_6, G-BOX BINDING FACTOR 5, GBF5, TRANSCRIPTION FACTOR GBF5, basic leucine-zipper 2, AT2G18160
basic leucine-zipper 2
GO Process (1)
GO Function (4)
GO Component (1)
Arabidopsis thaliana (Columbia)
PREY

BZIP25

ARABIDOPSIS THALIANA BASIC LEUCINE ZIPPER 25, ATBZIP25, BASIC LEUCINE ZIPPER O2 HOMOLOG 4, BZO2H4, basic leucine zipper 25, AT3G54620
basic leucine zipper 25
GO Process (2)
GO Function (3)
GO Component (1)
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Two-hybrid protein-protein interaction analysis in Arabidopsis protoplasts: establishment of a heterodimerization map of group C and group S bZIP transcription factors.

Ehlert A, Weltmeier F, Wang X, Mayer CS, Smeekens S, Vicente-Carbajosa J, Droege-Laser W

In vivo protein-protein interactions are frequently studied by means of yeast two-hybrid analysis. However, interactions detected in yeast might differ considerably in the plant system. Based on GAL4 DNA-binding (BD) and activation domains (AD) we established an Arabidopsis protoplast two-hybrid (P2H) system. The use of Gateway-compatible vectors enables the high-throughput screening of protein-protein interactions in plant cells. The efficiency of ... [more]

Plant J. Jun. 01, 2006; 46(5);890-900 [Pubmed: 16709202]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BZIP25 BZIP2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID